Subzero Services Offered

at Subzero Canine Reproduction

These are basic explanations to help us start you and your dog on the most successful breeding path!

At Subzero Reproduction, we are confident that you can TRUST us to offer you the absolute best in service and technology…the difference is in the details.

Please do not hesitate to call us with any questions so that we can discuss your dog’s particular breeding needs.

Our goal is to provide as much information as possible for each of our services here so you can understand what your dog may need and how we can help you. Each dog/bitch and breeding situation is different…one size DOES NOT FIT ALL so to speak. Contact us for more information and to discuss your options.

canine semen freezing cryopreservation - Subzero Canine Reproduction in Temecula California

Semen Cryopreservation (freezing) and Storage

At Subzero, our team specializes in semen cryopreservation (freezing) and storage for dog breeders. Also, our expertise lies in using various extenders, careful freezing methods, and proper storage procedures to ensure successful thawing, preservation, and breeding.

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canine semen freezing and storage solutions - Subzero Canine Reproduction in Temecula California

Semen Shipping (Cooled semen)

We offer the most economical and the safest possible way to ship cooled semen anywhere in the world. In addition, we offer multiple extenders as well. Not all dogs do well with the same extender.

All of our shipments get a full semen report from our AndroScope CASA (Computer Assisted Sperm Analysis) for you and your client so that you can be confident in your personal business transactions regarding your stud.

Progesterone Testing

Same day in-house blood test

Progesterone timing and testing is the single most helpful tool in a successful breeding and whelping. Timing is EVERYTHING when it comes to breeding and whelping dates.

We strongly encourage progesterone testing starting at 5-6 days from the first sign of your bitch being in season. Ideally, we like to see a pre-ovulation number, a number near ovulation, and a number confirming that your bitch followed through with ovulation and showed evidence that she continued to rise in progesterone.

So much weight is often put on numbers. However, it is important to understand that once your female has ovulated the numbers mean MUCH less. Timing from ovulation is KEY in planning breedings and the best breeding methods. We cannot express the importance of proper progesterone testing for your breeding success!

There are two different machines running at Subzero. Both are carefully calibrated regularly and are always up to date on routine maintenance done by laboratory technicians for those specific machines.

Our Idexx machine provides results in 15 minutes and can read progesterone levels up to 20.0 ng/mL. The cost for this test is $75.
Our Tosoh machine provides results in 1 hour and can read progesterone levels up to 40.0 ng/mL. The cost for this test is $65.

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Other Resources

Breeding Management and Ovulation Timing in the Bitch Idexx Progesterone Information Why 5 is not always the magic number

LH (Luteinizing Hormone) Testing

LH testing is under-utilized blood test that allows us the ability to further pinpoint the exact time of ovulation- THE MOST IMPORTANT DETAIL in breeding and often in planning for whelp.

LH test leaflet

AndroScope CASA (Computer Assisted Sperm Analysis)

We use the AndroScope CASA for all our semen evaluations stand alone or in conjunction with another service.

AndroScope is a compact mobile CASA system that is based on AndroVision® and allows for the analysis of semen samples anywhere.

This portable computer assisted sperm analysis is new technology and at the top of its class! It offers many advantages to you and your stud dog including its ability to chart your dog’s semen collection history.

Artificial Insemination

This includes and semen evaluation as well as proper AI technique.

Artificial insemination is a fairly simple procedure. However, we have sadly seen these go terribly wrong when attempted by inexperienced (and experienced) individuals. Unfortunately, they caused injury to a female or the end of her potential to breed. Please have your AI’s done by a licensed veterinary professional.

Transcerivical Insemination

TCI’s done well and correctly can be an invaluable breeding resource. It is much less invasive than a surgical implant where you must worry about all the dangers that go with anesthesia and full abdominal surgery. The Subzero team has more than two decades of experience performing transcervical inseminations. Also, we use the best scope in the business made by Karl Storz for optimal performance and success.

We invite your questions and will always allow you to be present so long as it is in the best interest of your pet.

A TCI is only as good as the technician or doctor performing it. The discrepancy of the skill level in individuals performing TCI’s is EXTREME. We consider ourselves some of the very best at this procedure and we have been involved in the training and education for local veterinarians and technicians.

x ray imaging - Subzero Canine Reproduction in Temecula California

Radiography (X-ray)

We are proud to offer state of the art Idexx radiology technology and equipment, which allows the clearest of images to be provided to our clientele. This is especially helpful when needing to determine final puppy count prior to whelp. The best time to perform a pregnancy radiograph is 55 days or later from breeding. An appointment is required for this service.

We are also proud to offer OFA (Orthopedic Foundation for Animals) image submissions. This is a requirement amongst many AKC breed clubs and may include several different image types such as:

- - Hips
  - Elbows
  - Trachea
  - Shoulder

Please call ahead to verify availability. In most cases, we are able to accommodate same day scheduling, but want to make sure we are able to dedicate all the time necessary to obtain excellent images.

X-ray price sheet

OFA (Orthopedic Foundation for Animals

We frequently receive inquiries regarding our success with proper positioning for OFA images, specifically for hips. Translating this success can be challenging, so we have included images of various breeds that we have taken. We take great pride in our success with these images and many others like them. As extreme perfectionists, we appreciate owners who understand that we strive for the best technique and outcome while minimizing stress and ensuring the comfort of your dog(s), particularly since all images taken here are done WITHOUT sedation.

We would like to extend our gratitude to all who choose to utilize our services for this purpose. We look forward to seeing you and your next generation of dogs on their health test journey!

OFA imaging is available Tuesday through Thursday. To schedule an appointment, please contact our office via phone or text at 951-587-9921.

For weekend appointments, please note that these are ONLY available during our OFA Health Clinic events in conjunction with the Cocker Spaniel Club of California. For more information on these events, please contact Celyn at K9healthclinics@gmail.com to inquire about upcoming clinics.

Please visit www.ofa.org for more information on specific breed diagnostic imaging

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We are confident that you can TRUST us to offer you the absolute best in service and technology…the difference is in the details.